Kontush A, Kohlschütter A, Beisiegel U
Medical Clinic, University of Hamburg, Germany.
Biochem Mol Biol Int. 1995 Nov;37(4):707-16.
Oxidation of four major plasma lipoproteins (chylomicrons, very low, low and high density lipoproteins) induced by Cu2+ was found to increase their mobility during agarose gel electrophoresis, their absorption between 220 and 280 nm and fluorescence at 360/430 nm. Oxidation decreased lipoprotein absorption between 400 and 500 nm, fluorescence at 290/330 nm and fluidity of lipoprotein lipids. Changing physical properties of the lipoproteins indicated that both their lipid and protein moieties were affected by oxidation. It is suggested that the relative contribution of lipid vs. protein oxidation to the oxidation of whole lipoproteins reflects their lipid/protein ratio, decreasing from chylomicrons to high density lipoproteins.
研究发现，铜离子诱导的四种主要血浆脂蛋白（乳糜微粒、极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白）氧化，会增加它们在琼脂糖凝胶电泳中的迁移率、220至280纳米之间的吸光度以及360/430纳米处的荧光。氧化会降低脂蛋白在400至500纳米之间的吸光度、290/330纳米处的荧光以及脂蛋白脂质的流动性。脂蛋白物理性质的改变表明，其脂质和蛋白质部分均受氧化影响。有人提出，脂质氧化与蛋白质氧化对整个脂蛋白氧化的相对贡献反映了它们的脂质/蛋白质比率，该比率从乳糜微粒到高密度脂蛋白逐渐降低。
