Steinberg M G, Chen I W, Silberstein E B
J Lab Clin Med. 1977 May;89(5):1105-11.
Autologous canine 131I-fibrinogen labeled by a chloramine-T method was examined to determine whether the fibrinogen had been altered by the iodination procedure as suggested by other investigators. Before and after radioiodination, clottability of the fibrinogen was always equal to or greater than 89 percent. Gel chromatography studies revealed that labeled fibrinogen behaved as unlabeled fibrinogen and no high molecular weight material was found. Gamma camera scintigraphy reflected the chromatography results, as iodinated material did not appear in the liver or spleen until 48 to 72 hours. The slow-moving component of the whole blood clearance curves, respresenting undenatured fibrinogen, had a mean half-life of 61.4 +/- 5.1 hours (+/- S.E.M.) and comprised 51.5 +/- 7.5 percent of the initial activity. Similarly, a mean falf-life of 53.4 +/- 2.0 hours and 59.6 +/- 7.7 percent initial activity wwere attributed to fibrinogen according to the plasma clearance curves as determined by trichloracetic acid precipitation. The results clearly demonstrated that chloramine-T iodination of fibrinogen can yield an excellent product, biologically and chemically, which is free from macroaggregates.
对用氯胺 - T法标记的自体犬131I - 纤维蛋白原进行了检测，以确定如其他研究者所指出的，纤维蛋白原是否因碘化过程而发生改变。在放射性碘化前后，纤维蛋白原的可凝性始终等于或大于89%。凝胶色谱研究表明，标记的纤维蛋白原表现与未标记的纤维蛋白原相同，未发现高分子量物质。γ相机闪烁扫描反映了色谱结果，因为碘化物质直到48至72小时才出现在肝脏或脾脏中。全血清除曲线中代表未变性纤维蛋白原的慢移动成分，其平均半衰期为61.4±5.1小时（±标准误），占初始活性的51.5±7.5%。同样，根据三氯乙酸沉淀法测定的血浆清除曲线，纤维蛋白原的平均半衰期为53.4±2.0小时，初始活性为59.6±7.7%。结果清楚地表明，纤维蛋白原的氯胺 - T碘化在生物学和化学上都能产生一种不含大聚集体的优质产物。
