Ishii T, Nishijima T
Forestry and Forest Products Research Institute, Ibaraki, Japan.
Plant Cell Physiol. 1995 Dec;36(8):1447-51.
The biological activity of cell wall-derived feruloyl oligosaccharides was investigated using a modified micro-drop bioassay. A feruloyl arabinoxylan trisaccharide (FAXX) and a feruloyl arabinoxylan tetrasaccharide (FAXXX) were found to inhibit the gibberellin-induced elongation of dwarf rice (Oryza sativa L., cv, Tan-ginbozu) that had been treated with uniconazole (S-3307), an inhibitor of the biosynthesis of gibberellins. An arabinoxylan trisaccharide (AXX) was ineffective. The growth-inhibitory effect of feruloyl oligosaccharides depended on their feruloyl and glycosyl moieties. The amount of esterified diferulic acid residues in the cell walls of the second leaf sheath of rice seedlings that had been treated with FAXX was almost same as that of controls. Feruloyl oligosaccharides did not inhibit the incorporation of [14C]leucine into acid-precipitable proteins by suspension-cultured maize cells, whereas cinnamic acid and its derivatives strongly inhibited such incorporation.
采用改良的微量滴注生物测定法研究了细胞壁衍生阿魏酰低聚糖的生物活性。发现一种阿魏酰阿拉伯木聚糖三糖（FAXX）和一种阿魏酰阿拉伯木聚糖四糖（FAXXX）可抑制赤霉素诱导的矮化水稻（水稻品种“Tan-ginbozu”）的伸长，该矮化水稻已用赤霉素生物合成抑制剂烯效唑（S-3307）处理过。一种阿拉伯木聚糖三糖（AXX）则无此效果。阿魏酰低聚糖的生长抑制作用取决于其阿魏酰基和糖基部分。用FAXX处理过的水稻幼苗第二叶鞘细胞壁中酯化二阿魏酸残基的含量与对照几乎相同。阿魏酰低聚糖不抑制悬浮培养的玉米细胞将[14C]亮氨酸掺入酸沉淀蛋白中，而肉桂酸及其衍生物则强烈抑制这种掺入。
