Sakamoto K, Shimomura K, Komeda Y, Kamada H, Satoh S
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Plant Cell Physiol. 1995 Dec;36(8):1549-54.
Male-associated DNA sequences were analyzed in a dioecious plant, Cannabis sativa L. (family: Moraceae), which is known to have sex chromosomes. DNA was isolated from male and female plants and subjected to random amplification of polymorphic DNA. Two out of 15 primers yielded fragments of 500 and 730 bp which were detected in all male plants but not in any of the female plants tested. These two DNA fragments were cloned and used as probes in gel blot analysis of genomic DNA. When the male and female DNAs were allowed to hybridize with the 500-bp probe, no differences in patterns were observed between male and female plants. By contrast, when these DNAs were allowed to hybridize with the 730-bp probe, much more intense bands specific to male plants were detected, in addition to less intense bands that were common to both sexes. The 730-bp DNA fragment was named MADC1 (male-associated DNA sequence in Cannabis sativa). The sequence of MADC1 did not include a long open reading frame and it exhibited no significant similarity to previously reported sequences.
在一种雌雄异株的植物大麻（大麻科）中分析了与雄性相关的DNA序列，已知该植物具有性染色体。从雄性和雌性植株中分离出DNA，并进行多态性DNA的随机扩增。15种引物中有两种产生了500和730 bp的片段，在所有雄性植株中都能检测到，但在所测试的任何雌性植株中都未检测到。将这两个DNA片段克隆，并用作基因组DNA凝胶印迹分析的探针。当雄性和雌性DNA与500 bp的探针杂交时，未观察到雄性和雌性植株之间的模式差异。相比之下，当这些DNA与730 bp的探针杂交时，除了两性共有的较弱条带外，还检测到了雄性植株特有的更强条带。730 bp的DNA片段被命名为MADC1（大麻中与雄性相关的DNA序列）。MADC1的序列不包含长开放阅读框，并且与先前报道的序列没有显著相似性。
