Willems A, Collins M D
Department of Microbiology, Institute of Food Research, Reading Laboratory, United Kingdom.
Int J Syst Bacteriol. 1995 Jul;45(3):572-5. doi: 10.1099/00207713-45-3-572.
The 16S rRNA gene sequence of the type strain of Ruminococcus flavefaciens, the type species of the genus Ruminococcus, was determined by PCR direct sequencing. A comparative sequence analysis showed that R. flavefaciens is phylogenetically related to a small cluster (cluster IV of Collins et al. [M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994]) of organisms which includes several Clostridium and Eubacterium species. R. flavefaciens was found to be phylogenetically only remotely related to Ruminococcus gnavus, Ruminococcus torques, Peptostreptococcus productus, and Streptococcus hansenii. These findings demonstrate that the genus Ruminococcus is not a monophyletic group, and the proposed transfer of P. productus and S. hansenii to this genus (T. Ezaki, N. Li, Y. Hashimoto, H. Miura, and H. Yamamoto, Int. J. Syst. Bacteriol. 44:130-136, 1994) is not supported.
通过PCR直接测序法测定了瘤胃球菌属模式种黄化瘤胃球菌模式菌株的16S rRNA基因序列。比较序列分析表明，黄化瘤胃球菌在系统发育上与一小类菌（Collins等人[M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994]的IV簇）相关，该类菌包括几个梭菌属和真杆菌属的菌种。发现黄化瘤胃球菌在系统发育上与纤细瘤胃球菌、扭链瘤胃球菌、产物消化链球菌和汉氏链球菌的关系较远。这些发现表明瘤胃球菌属不是一个单系群，因此不支持将产物消化链球菌和汉氏链球菌转入该属的提议（T. Ezaki, N. Li, Y. Hashimoto, H. Miura, and H. Yamamoto, Int. J. Syst. Bacteriol. 44:130-136, 1994）。
