Lichko L P, Kulakovskaia T V, Dmitriev V V, Kulaev I S
Biokhimiia. 1995 Nov;60(11):1911-6.
Intact nuclei have been isolated from cells of a diploid strain of Saccharomyces cerevisiae. The isolated nuclei were free from cytoplasmic, mitochondrial and vacuolar marker enzymes. The protein to DNA ratio (w/w) was 11. Pyrophosphatase, tripolyphosphatase and exopolyphosphatase activities have been found in S. cerevisiae nuclei for the first time and were equal to 400, 130 and 55 mU/mg of protein, respectively.
已从酿酒酵母二倍体菌株的细胞中分离出完整的细胞核。分离出的细胞核不含细胞质、线粒体和液泡标记酶。蛋白质与DNA的比例（重量/重量）为11。首次在酿酒酵母细胞核中发现了焦磷酸酶、三聚磷酸酶和外聚磷酸酶活性，分别为400、130和55 mU/mg蛋白质。
