Haight M, Davidson D, Pasternak J
J Parasitol. 1977 Apr;63(2):267-73.
During the proliferative phase of cercarial development in the digenetic trematode Trichobilharzia ocellata, nuclei varied in size, appearance of the chromatin, and intensity of Feulgen staining. On the basis of interphase nuclear morphology six nuclear classes were identified. Data from microspectrophotometric determinations and 3H-TdR labeling experiments were used to correlate each kind of interphase nucleus with a phase of the cell cycle. Marked variability in nuclear area developed between cells by time they reached late G1. Increases in nuclear area could not be correlated with the onset of DNA synthesis. Throughout all stages of development of T. ocellata cercariae, proliferating cells divide mitotically; meiotic divisions were never seen. Thus, the mode of reproduction appears to be asexual. No evidence was found to support previous suggestions of diploid parthenogenesis.
在复殖吸虫眼点毛毕吸虫尾蚴发育的增殖阶段，细胞核在大小、染色质外观以及福尔根染色强度方面存在差异。基于间期核形态鉴定出了六种类别。利用显微分光光度测定数据和3H - 胸腺嘧啶核苷标记实验，将每种间期核与细胞周期的一个阶段进行关联。在细胞进入G1晚期时，细胞间核面积出现显著差异。核面积的增加与DNA合成的起始并无关联。在眼点毛毕吸虫尾蚴发育的所有阶段，增殖细胞进行有丝分裂；从未观察到减数分裂。因此，其繁殖方式似乎是无性的。未找到证据支持之前关于二倍体孤雌生殖的推测。
