Peyrin E, Guillaume Y, Guinchard C
Laboratoire de Chimie analytique, UFR des Sciences Médicales et Pharmaceutiques, Besancon, France.
J Chromatogr B Biomed Appl. 1995 Oct 6;672(1):160-4. doi: 10.1016/0378-4347(95)00193-m.
A new reversed-phase high-performance liquid chromatography (RP-HPLC) method for the detection and quantification of tazobactam in serum and haemofiltration fluid is described. The assay for these biological fluids involves an extraction with diethyl ether followed by derivatization using 1,2,4-triazole. The mobile phase consisted of phosphate buffer-methanol and the detection wavelength was 325 nm. The limit of detection was 0.05 microgram/ml in the two fluids and the calibration curves were linear over the range 0.1-50 micrograms/ml. For a tazobactam concentration equal to 1, 5 or 20 micrograms ml-1, the coefficients of variation were less than 5%. The assay was successfully applied to the analysis of samples from drug monitoring in a patient with renal insufficiency undergoing continuous venovenous haemofiltration (CVVH).
描述了一种用于检测和定量血清及血液滤过液中他唑巴坦的新型反相高效液相色谱（RP-HPLC）方法。这些生物流体的测定方法包括用乙醚萃取，然后用1,2,4-三唑进行衍生化。流动相由磷酸盐缓冲液-甲醇组成，检测波长为325nm。两种流体中的检测限均为0.05微克/毫升，校准曲线在0.1-50微克/毫升范围内呈线性。对于他唑巴坦浓度等于1、5或20微克/毫升的情况，变异系数小于5%。该测定方法已成功应用于对一名接受持续静脉-静脉血液滤过（CVVH）的肾功能不全患者进行药物监测的样本分析。
