Peterson R J, Kwedar S A, Moticka E J
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62708, USA.
Invest Ophthalmol Vis Sci. 1987 Feb;28(2):281-6.
Intralamellar keratoplasty in inbred rats is a valuable method for the investigation of corneal allograft rejection. Present methods of intralamellar corneal grafting are tedious and time-consuming. In addition, induction of neovascularization, a prerequisite to inducing graft rejection, is variable. In an effort to overcome these problems, the authors have developed an improved method of intralamellar keratoplasty. Intralamellar pockets are formed by introducing a 30-g needle into the corneal stroma near the limbus. Approximately 10-25 microliters of lipopolysaccharide (LPS) (100 micrograms/ml) is injected into the stroma, forming a stromal bleb. This bleb is incised to form a pocket, resulting in the leakage of the injected liquid. The intralamellar pocket formation is completed by dissecting any remaining stromal fibers in the area of former bleb. Once the pocket has been formed, grafts of corneal tissue are inserted, and the incision is closed. This method of keratoplasty has the following advantages over previously reported methods: (1) It is more rapid and less tedious because the formation of the corneal bleb protects the anterior chamber from being invaded during the corneal incision; (2) It leads to a reproducible induction of neovascularization in every cornea so treated; (3) It results in a higher frequency of allograft rejection.
近交系大鼠板层角膜移植术是研究角膜移植排斥反应的一种有价值的方法。目前的板层角膜移植方法繁琐且耗时。此外，诱导新生血管形成（诱导移植排斥反应的一个先决条件）的效果存在差异。为了克服这些问题，作者开发了一种改良的板层角膜移植术方法。通过将一根30号针头插入角膜缘附近的角膜基质来形成板层袋。将大约10 - 25微升脂多糖（LPS）（100微克/毫升）注入基质，形成一个基质泡。切开这个泡以形成一个袋，导致注入的液体渗漏。通过解剖先前泡区域内任何残留的基质纤维来完成板层袋的形成。一旦袋形成，将角膜组织移植物插入，并关闭切口。这种角膜移植术方法与先前报道的方法相比具有以下优点：（1）它更快且不那么繁琐，因为角膜泡的形成可保护前房在角膜切开过程中不被侵入；（2）它能在每只接受治疗的角膜中可重复地诱导新生血管形成；（3）它导致同种异体移植排斥反应的频率更高。
