Bragadin M, Dell'Antone P
Department of Environmental Sciences, University of Venice, Italy.
Arch Environ Contam Toxicol. 1996 Feb;30(2):280-4. doi: 10.1007/BF00215809.
This paper examines the accumulation and toxicity mechanism of a cationic detergent, cetyltrimethylammonium (bromide) (CTAB), in energized rat liver mitochondria. The results suggest that: (1) the CTAB ion is accumulated in the mitochondrial matrix by a membrane potential-driven uptake mechanism; (2) accumulation may lead to a toxic effect, since it gives rise to collapse of the mitochondrial membrane potential (delta phi) which is correlated with ATP synthesis and which regulates Ca++ uptake; (3) collapse of delta phi may be due to enhanced permeability of the mitochondrial membrane to the ions (detergent effect); and (4) delta phi collapse and Ca++ and K+ release were also observed in another cationic detergent, NTAB, but not in the presence of anionic detergents.
本文研究了阳离子去污剂十六烷基三甲基溴化铵（CTAB）在供能大鼠肝线粒体中的积累及毒性机制。结果表明：（1）CTAB离子通过膜电位驱动的摄取机制积累在线粒体基质中；（2）积累可能导致毒性作用，因为它会引起与ATP合成相关且调节Ca++摄取的线粒体膜电位（Δφ）的崩溃；（3）Δφ的崩溃可能是由于线粒体膜对离子的通透性增强（去污剂效应）；（4）在另一种阳离子去污剂NTAB中也观察到了Δφ的崩溃以及Ca++和K+的释放，但在阴离子去污剂存在的情况下未观察到。
