[Preparation and separation of milligram amounts of canine fibrin(ogen) degradation products (fdp) X, Y, D and E].

In the present investigation we first produced canine fibrinogen degradation products (FDP) following two optimized degradation protocols. These FDP-mixtures, which were alternatively enriched with X and Y fragments or D and E fragments, were purified further to individual FDP X, -Y, -D, and -E with > 95% purity by the means of two low pressure column chromatographic techniques (size exclusion chromatography and anionexchanger chromatography). With this techniques the FDP D could be separated into four different D subfractions. No satisfactory results were yielded by hydrophobic interaction chromatography (HIC) with C5-Alkylsuperose, chromato-focusing and separations with hydroxyapatit. The observed strong binding of fragment E on hydroxyapatit probably points to the maintenance of the calcium binding site on the prepared canine E-fragment.