Hirata S, Mori Y
Japanese Wildlife Research Center, Tokyo, Japan.
J Vet Med Sci. 1995 Oct;57(5):845-50. doi: 10.1292/jvms.57.845.
Characterization of the estrous cycle and the ability to detect pregnancy are essential for the development of efficient captive breeding programs in wild mammalian species and for their conservation. We assess here the feasibility of using fecal progesterone analysis as a non-invasive method of monitoring the ovarian function of the Shiba goat as an experimental model for ruminant species. Feces and blood samples were collected sequentially from 4 female goats throughout the estrous cycle and gestation period and from 2 ovariectomized goats which were subcutaneously implanted with progesterone. The recovery rate with ether extraction was about 70% for fecal progesterone. Fecal progesterone concentrations changed dramatically in accordance with plasma progesterone during the estrous cycle and the pregnancy. Moreover, implantation and removal of progesterone capsules resulted in matched elevation and decline in fecal and plasma progesterone. The present data suggest that measurement of progesterone in feces is a potential noninvasive method of assessing the reproductive status of ruminant species.
表征发情周期以及检测怀孕的能力对于野生哺乳动物高效圈养繁殖计划的制定及其保护至关重要。我们在此评估使用粪便孕酮分析作为一种非侵入性方法来监测柴山羊卵巢功能的可行性，柴山羊作为反刍动物物种的实验模型。在整个发情周期和妊娠期，从4只雌性山羊以及2只皮下植入孕酮的去卵巢山羊中依次采集粪便和血液样本。粪便孕酮的乙醚提取回收率约为70%。在发情周期和怀孕期间，粪便孕酮浓度随血浆孕酮浓度发生显著变化。此外，孕酮胶囊的植入和取出导致粪便和血浆孕酮相应升高和下降。目前的数据表明，测量粪便中的孕酮是评估反刍动物生殖状态的一种潜在非侵入性方法。
