Ferris N P, Oxtoby J M, Hughes J F
Biotechnology and Biological Sciences Research Council, Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Rev Sci Tech. 1995 Sep;14(3):557-65. doi: 10.20506/rst.14.3.859.
The current routine diagnostic procedures for foot and mouth disease (FMD) virus antigen detection combine the use of an indirect sandwich enzyme-linked immunosorbent assay (ELISA) with virus isolation and amplification on cell culture. Several field samples recently received by the World Reference Laboratory for Foot and Mouth Disease which were initially diagnosed as containing a single virus type have subsequently been found to contain an additional virus type. Examples are given of the results of ELISAs performed on certain Saudia Arabian samples; these examples illustrate the problem which such multiple-infected samples present for laboratory diagnosis.
目前口蹄疫病毒抗原检测的常规诊断程序是将间接夹心酶联免疫吸附测定（ELISA）与在细胞培养上进行病毒分离和扩增相结合。世界口蹄疫参考实验室最近收到的几份野外样本，最初被诊断为只含有一种病毒类型，但随后发现还含有另一种病毒类型。文中给出了对某些沙特阿拉伯样本进行ELISA检测的结果示例；这些示例说明了此类多重感染样本给实验室诊断带来的问题。
