Lalles J P, Benkredda D, Toullec R
Laboratoire du Jeune Ruminant, Institut National de la Recherche Agronomique-Ecole, Nationale Supérieure Agronomique, Rennes, France.
Zentralbl Veterinarmed A. 1995 Sep;42(7):467-78. doi: 10.1111/j.1439-0442.1995.tb00401.x.
An experiment was designed to determine the soya antigen levels in milk replacers above which gastrointestinal disorders appeared in preruminant calves previously sensitized to antigenic soya by feeding a soya-based milk replacer for 3 months. These calves were then equipped with wire electrodes on the duodenum and the mid-jejunum. The sensitization was visualized using direct skin testing, plasma anti-soya antibody determination and intestinal myoelectric activity recording. After sensitization, the calves were occasionally fed liquid test meals containing various proportions of antigenic soya and whey. The soya-fed calves displayed larger 24 h skin reactions to beta-conglycinin and higher plasma anti-soya antibody titres than the controls maintained on a skim-milk based milk replacer. Disturbances of the myoelectric activity patterns were recorded on the duodenum and mid-jejunum after feeding antigenic soya, but not non-antigenic soya or milk protein, in the soya-sensitized calves. When the level of antigens was varied, disorders in the jejunal motility patterns appeared when antigenic soya provided one-third or more of the dietary protein in the test meals, although some abnormalities were evident at lower incorporation rates. The major change was a reduction in the mean duration of the jejunal migrating motor complexes which was essentially accounted for by a decrease in the mean duration of the phase I (quiescence). This level of antigens corresponded approximately to 14 and 12 mg of immunoreactive glycinin and beta-conglycinin respectively, per gram of protein intake, i.e. 80 and 70 mg/kg0.75 per test meal.
设计了一项实验，以确定代乳粉中的大豆抗原水平，超过该水平时，此前通过喂食基于大豆的代乳粉3个月而对大豆抗原致敏的反刍前犊牛会出现胃肠道疾病。然后在这些犊牛的十二指肠和空肠中部安装电极丝。通过直接皮肤试验、血浆抗大豆抗体测定和肠肌电活动记录来观察致敏情况。致敏后，偶尔给犊牛喂食含有不同比例抗原性大豆和乳清的液体试验餐。与以脱脂乳为基础的代乳粉喂养的对照组相比，喂食大豆的犊牛对β-伴大豆球蛋白的24小时皮肤反应更大，血浆抗大豆抗体滴度更高。在喂食抗原性大豆后，在致敏大豆的犊牛的十二指肠和空肠中部记录到肌电活动模式的紊乱，但喂食非抗原性大豆或乳蛋白时未出现这种情况。当抗原水平变化时，当抗原性大豆在试验餐中提供三分之一或更多的膳食蛋白质时，空肠蠕动模式出现紊乱，尽管在较低的掺入率下也有一些异常明显。主要变化是空肠移行性运动复合波的平均持续时间缩短，这主要是由于I期（静止期）的平均持续时间缩短所致。该抗原水平分别相当于每克蛋白质摄入量约14毫克和12毫克免疫反应性大豆球蛋白和β-伴大豆球蛋白，即每份试验餐80毫克/千克0.75和70毫克/千克0.75。
