Bernatzky R, Glaven R H, Rivers B A
Department of Plant and Soil Sciences, University of Massachusetts, Amherst 01003, USA.
Biochem Genet. 1995 Aug;33(7-8):215-25.
Stylar proteins involved in the self-incompatible (SI) response of Lycopersicon hirsutum have been identified and mapped to the locus that controls SI (S locus). L. esculentum, a self-compatible (SC) species of cultivated tomato, does not display these proteins. Hybrids between SC L. esculentum and SI L. hirsutum are self-sterile despite these individuals bearing pollen containing the S allele of L. esculentum. In progeny derived from backcrossing the hybrids to L. esculentum, there was a strong correlation between the presence of the S allele from L. hirsutum and self-infertility. However, this relationship was uncoupled in a number of backcross (BC) progeny. The SI response appeared to be nonexistent in two self-fertile BC individuals that were heterozygous for the S allele of L. hirsutum, based on Mendelian segregation of a tightly linked DNA marker, CD15, in selfed progeny. Among these progeny self-fertile individuals that were homozygous for the L. hirsutum allele of the linked marker were also determined to be homozygous for an S-related proteins are not sufficient to elicit a self-incompatible response in L. esculentum and that there is a mutation(s) in L. esculentum somewhere other than the S locus that leads to self-compatibility.
参与多毛番茄自交不亲和（SI）反应的花柱蛋白已被鉴定，并定位到控制SI的位点（S位点）。栽培番茄的自交亲和（SC）物种——普通番茄，不表现出这些蛋白。尽管SC普通番茄与SI多毛番茄的杂种个体携带含有普通番茄S等位基因的花粉，但它们是自交不育的。在将杂种与普通番茄回交产生的后代中，来自多毛番茄的S等位基因的存在与自交不育之间存在很强的相关性。然而，这种关系在一些回交（BC）后代中被打破。根据紧密连锁的DNA标记CD15在自交后代中的孟德尔分离情况，两个对多毛番茄S等位基因杂合的自交可育BC个体似乎不存在SI反应。在这些后代中，与连锁标记的多毛番茄等位基因纯合的自交可育个体也被确定为与一种S相关蛋白纯合，这表明这些蛋白不足以在普通番茄中引发自交不亲和反应，并且在普通番茄中除S位点之外的其他某个位置存在导致自交亲和的突变。
