Guthrie K M, Gall C M
Department of Anatomy and Neurobiology, University of California, Irvine 92717, USA.
Neuroreport. 1995 Nov 13;6(16):2145-9; discussion 2103. doi: 10.1097/00001756-199511000-00012.
Neuronal activity may lead to long lasting changes in cell phenotype through induction of genes such as c-fos which encode transcriptional regulatory factors. Odor-activated olfactory bulb cells exhibit increases in c-fos mRNA expression. The present study examined whether odor stimulation of awake rats also leads to increases in Fos protein in these cells. The phenotype of Fos-immunoreactive cells was partially characterized using double-immunoperoxidase staining. Odor exposure increased Fos-immunoreactivity (IR) in specific sets of olfactory bulb neurons. Fos-IR was not co-localized with IR for glial fibrillary acidic protein, but was co-localized with tyrosine hydroxylase (TH)-IR in a subpopulation of dopaminergic neurons, suggesting that bulbar TH expression may be regulated in part by a Fos mechanism.
神经元活动可能通过诱导如c-fos等编码转录调节因子的基因，导致细胞表型发生长期变化。气味激活的嗅球细胞中c-fos mRNA表达增加。本研究检测了清醒大鼠的气味刺激是否也会导致这些细胞中Fos蛋白增加。使用双重免疫过氧化物酶染色对Fos免疫反应性细胞的表型进行了部分表征。气味暴露增加了嗅球特定神经元组中的Fos免疫反应性（IR）。Fos-IR与胶质纤维酸性蛋白的IR不共定位，但在多巴胺能神经元亚群中与酪氨酸羟化酶（TH）-IR共定位，这表明球部TH表达可能部分受Fos机制调节。
