Tremblay M, Salin P, Soghomonian J J
Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Québec, Canada.
Neuroreport. 1995 Nov 13;6(16):2225-9. doi: 10.1097/00001756-199511000-00030.
Levels of mRNAs encoding for subunits of glutamate receptors were measured by in situ hybridization in the rat striatum after unilateral injections of 6-hydroxydopamine in the substantia nigra. When compared with the contralateral striatum, levels of NMDAR1, GluR1 and GluR2 mRNAs were significantly higher in the dopamine-deafferented striatum. Comparison with saline-injected rats showed that the NMDAR1 and the GluR1 mRNA labelling was increased in the striatum ipsilateral to the lesion. In contrast, GluR2 mRNA labelling in 6-OHDA-injected rats was not different from that in saline-injected rats. These results support the hypothesis that experimental dopamine deafferentation induces functional changes at the level of glutamatergic synapses in the striatum.
在大鼠黑质单侧注射6-羟基多巴胺后，通过原位杂交法测定了纹状体中编码谷氨酸受体亚基的mRNA水平。与对侧纹状体相比，多巴胺去传入的纹状体中NMDAR1、GluR1和GluR2 mRNA水平显著更高。与注射生理盐水的大鼠相比，损伤同侧纹状体中的NMDAR1和GluR1 mRNA标记增加。相反，注射6-OHDA的大鼠中GluR2 mRNA标记与注射生理盐水的大鼠无差异。这些结果支持了实验性多巴胺去传入诱导纹状体谷氨酸能突触水平功能变化这一假说。
