Wieser R J, Baumann C E, Oesch F
Institute of Toxicology, Mainz, Germany.
Glycoconj J. 1995 Oct;12(5):672-9. doi: 10.1007/BF00731264.
Contactinhibin was found to be involved in contact-dependent inhibition of growth. The growth inhibitory activity of contactinhibin is mediated by N-linked oligosaccharides with desialylated beta-glycosidically linked, terminal galactose residues. Here we show that in sparse human fibroblasts contactinhibin was expressed in a biologically inactive, highly sialylated form both on the plasma membrane and intracellularly, while in confluent cells plasma membrane localized contactinhibin was present in a biologically active, low sialylated form. Plasma membranes were shown to contain a glycoprotein sialidase which is suggested to be engaged in the activation of contactinhibin in a cell contact-dependent manner.
发现接触抑制素参与接触依赖性生长抑制。接触抑制素的生长抑制活性由具有去唾液酸化的β-糖苷键连接的末端半乳糖残基的N-连接寡糖介导。在此我们表明，在稀疏的人成纤维细胞中，接触抑制素以生物无活性、高度唾液酸化的形式在质膜和细胞内表达，而在汇合细胞中，质膜定位的接触抑制素以生物活性、低唾液酸化的形式存在。已表明质膜含有一种糖蛋白唾液酸酶，提示其以细胞接触依赖性方式参与接触抑制素的激活。
