Höltke H J, Ankenbauer W, Mühlegger K, Rein R, Sagner G, Seibl R, Walter T
Boehringer Mannheim GmbH, Dept. of Molecular Biology, Penzberg, Germany.
Cell Mol Biol (Noisy-le-grand). 1995 Nov;41(7):883-905.
The use of non-radioactive nucleic acid probes has increased dramatically over the last years. The convenience of not having to deal with radioactive isotopes combined with the stability and economy of the non-radioactive systems has led to applications in various techniques. One of the most successful labelling and detection systems is based on the hapten digoxigenin. Here, the different methods for labelling nucleic acids with digoxigenin as well as the various possibilities for detection are described. Some typical applications illustrate the utility of the DIG system.
在过去几年中，非放射性核酸探针的使用急剧增加。无需处理放射性同位素的便利性，再加上非放射性系统的稳定性和经济性，使得其在各种技术中得到了应用。最成功的标记和检测系统之一是基于半抗原地高辛。本文描述了用地高辛标记核酸的不同方法以及各种检测可能性。一些典型应用说明了地高辛系统的实用性。
