Takeda M, Hosoyamada M, Shirato I, Obinata M, Suzuki M, Endou H
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Biochem Mol Biol Int. 1995 Oct;37(3):507-15.
A little is known about vasopressin receptor in early proximal tubule (S1). The purpose of this study is to establish the vasopressin-responsive S1 cell line derived from transgenic mice harboring temperature-sensitive(ts) simian virus (SV) 40 large T-antigen gene. The cells showed a temperature-sensitive cell growth characteristic of encoding tsSV40. The S1 cells retained a unique morphology specific to proximal tubule. The cells showed the vasopressin-induced increase in intracellular calcium concentration ([Ca2+]i) mediated by both V1a and the putative Vp receptor. Of nineteen clonal cell lines established from the parental cells, three expressed only V1a receptor, and five retained both V1a and Vp receptor. In conclusion, these immortalized S1 cell lines may be useful for studying vasopressin receptor subtypes in S1.
关于早期近端小管（S1）中的血管加压素受体，人们了解甚少。本研究的目的是建立源自携带温度敏感（ts）猿猴病毒（SV）40大T抗原基因的转基因小鼠的血管加压素反应性S1细胞系。这些细胞表现出编码tsSV40的温度敏感细胞生长特性。S1细胞保留了近端小管特有的独特形态。这些细胞显示血管加压素诱导的细胞内钙浓度（[Ca2+]i）增加，这是由V1a和假定的Vp受体介导的。从亲代细胞建立的19个克隆细胞系中，3个仅表达V1a受体，5个同时保留V1a和Vp受体。总之，这些永生化的S1细胞系可能有助于研究S1中的血管加压素受体亚型。
