Christiansen S K, Knudsen S, Giese H
Plant Genetics Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000 Roskilde, Denmark.
Curr Genet. 1995 Dec;29(1):100-2. doi: 10.1007/BF00313200.
Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces. GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei β-tubulin promoter. Three heterologous promoters, onefrom Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.
粒子枪加速似乎是转化生长在寄主表面的专性植物寄生物菌丝体细胞的一种可能方法。在用大麦白粉菌β-微管蛋白启动子控制下的GUS基因轰击后，在大麦白粉菌细胞中获得了GUS表达。三个异源启动子，一个来自构巢曲霉，两个来自玉米小斑病菌，GUS表达非常低或无表达。
