Messmer T O, Black C M, Facklam R R
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Clin Biochem. 1995 Dec;28(6):567-72. doi: 10.1016/0009-9120(95)00044-0.
In the clinical laboratory, identification of Streptococcus pneumoniae can be confused with other streptococci. Conventional biochemical tests such as optochin sensitivity and bile solubility can give inconsistent results. This report presents a method to distinguish true S. pneumoniae from other upper respiratory tract streptococci when conventional tests fail.
We used arbitrarily primed polymerase chain reaction with the single primer M13 universal as a method to distinguish S. pneumoniae from other upper respiratory tract streptococci.
The fingerprint pattern of S. pneumoniae was established by amplifying DNA of S. pneumoniae type strains 1-48 and of other common upper respiratory tract streptococci at three different DNA concentrations with the single primer M13 universal. From these type strains, a common arbitrarily primed-polymerase chain reaction pattern was identified characterized by two predominant bands of equal intensity at 800 base pairs and at 1100 base pairs. Fingerprint patterns of viridans streptococci were easily distinguishable from those of S. pneumoniae. Many of the clinical isolates used in this study were equivocal by conventional tests but were distinguishable by their fingerprint patterns.
Our results indicated that the fingerprint pattern of S. pneumoniae is species specific and distinguishes true S. pneumoniae of clinical isolates from other streptococci when conventional biochemical tests are unclear.
在临床实验室中，肺炎链球菌的鉴定可能会与其他链球菌混淆。传统的生化试验，如奥普托欣敏感性试验和胆汁溶解试验，可能会得出不一致的结果。本报告介绍了一种在传统试验失败时，将真正的肺炎链球菌与其他上呼吸道链球菌区分开来的方法。
我们使用单引物M13通用引物的任意引物聚合酶链反应作为区分肺炎链球菌与其他上呼吸道链球菌的方法。
通过用单引物M13通用引物在三种不同DNA浓度下扩增1-48型肺炎链球菌菌株和其他常见上呼吸道链球菌的DNA，建立了肺炎链球菌的指纹图谱。从这些菌株中，鉴定出一种常见的任意引物聚合酶链反应图谱，其特征是在800个碱基对和1100个碱基对处有两条强度相等的主要条带。草绿色链球菌的指纹图谱很容易与肺炎链球菌的指纹图谱区分开来。本研究中使用的许多临床分离株通过传统试验难以明确，但通过其指纹图谱可以区分。
我们的结果表明，肺炎链球菌的指纹图谱具有种属特异性，当传统生化试验结果不明确时，可将临床分离株中的真正肺炎链球菌与其他链球菌区分开来。
