小鼠A3腺苷受体基因Adora3的染色体定位

Chromosomal mapping of the mouse A3 adenosine receptor gene, Adora3.

作者信息

Zhao Z, Ravid S, Ravid K

机构信息

Department of Biochemistry, Boston University School of Medicine, MA 02118, USA.

出版信息

Genomics. 1995 Nov 1;30(1):118-9. doi: 10.1006/geno.1995.0023.

DOI:10.1006/geno.1995.0023

PMID:8595892

Abstract

A3 adenosine receptor is a member of Gi protein-coupled receptors that mediate inhibition of adenylate cyclase activity upon binding to the ligand. We determined the chromosome localization of the mouse A3 gene for future genetic studies, utilizing an interspecific backcross panel formed from the cross (C57BL/6J x Mus spretus) F1 x M. spretus. Genomic DNAs from 94 individuals in the backcross were analyzed by Southern hybridization with murine A3 receptor cDNA probe. Unique map positions were determined by haplotype analysis with 1388 previously mapped loci in the mouse backcross. The mouse A3 receptor gene (Adora3) mapped to chromosome 3, in tight linkage with DNA marker D3Bir15.

摘要

A3腺苷受体是Gi蛋白偶联受体家族的成员，与配体结合后可介导腺苷酸环化酶活性的抑制。为了未来的遗传学研究，我们利用由（C57BL/6J×小家鼠）F1与小家鼠杂交形成的种间回交群体，确定了小鼠A3基因的染色体定位。用小鼠A3受体cDNA探针通过Southern杂交分析回交群体中94个个体的基因组DNA。通过对小鼠回交群体中1388个先前定位的基因座进行单倍型分析，确定了独特的图谱位置。小鼠A3受体基因（Adora3）定位于3号染色体，与DNA标记D3Bir15紧密连锁。