Laing T J, Richardson B C, Toth M B, Smith E M, Marks R M
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, USA.
J Rheumatol. 1995 Nov;22(11):2126-31.
Having previously found that treating small areas of synovitis within the knees of patients with rheumatoid arthritis (RA) with 8-methoxypsoralen (8-MOP) and laser-derived ultraviolet A (PUVA) resulted in decreases in adhesion molecule expression, we sought to determine the effect of PUVA on expression of vascular cellular adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), and E-selection by human umbilical vein endothelial cells (HUVEC).
Expression of VCAM-1, ICAM-1, and E-selectin on the surface of HUVEC was measured using specific antibodies and flow cytometry or a fluorescence plate reader, following treatment of cells with 8-MOP and UVA, before and after tumor necrosis factor (TNF) stimulation.
PUVA led to significant dose dependent decreases in the expression of VCAM-1 and E-selectin that had been induced with TNF before PUVA treatment. Pretreatment with PUVA was also able to prevent subsequent TNF induction of VCAM-1 expression. TNF-induced ICAM-1 expression was not decreased by PUVA, however, and pretreatment only partially decreased ICAM-1 expression.
The in vivo effects of PUVA may be explained, in part, by down regulation of adhesion molecule expression. The relative resistance of ICAM-1 to PUVA suggests some specificity to the effect on adhesion molecule expression.
先前发现用8-甲氧基补骨脂素（8-MOP）和激光衍生的紫外线A（PUVA）治疗类风湿性关节炎（RA）患者膝关节内的小面积滑膜炎会导致黏附分子表达降低，我们试图确定PUVA对人脐静脉内皮细胞（HUVEC）血管细胞黏附分子1（VCAM-1）、细胞间黏附分子1（ICAM-1）和E-选择素表达的影响。
在用8-MOP和紫外线A处理细胞后，在肿瘤坏死因子（TNF）刺激前后，使用特异性抗体和流式细胞术或荧光酶标仪测量HUVEC表面VCAM-1、ICAM-1和E-选择素的表达。
PUVA导致在PUVA处理前由TNF诱导的VCAM-1和E-选择素表达显著剂量依赖性降低。PUVA预处理也能够阻止随后TNF诱导的VCAM-1表达。然而，TNF诱导的ICAM-1表达并未因PUVA而降低，并且预处理仅部分降低了ICAM-1表达。
PUVA的体内作用可能部分通过黏附分子表达的下调来解释。ICAM-1对PUVA的相对抗性表明对黏附分子表达的影响具有一定特异性。
