一种与RNA聚合酶II全酶相关的哺乳动物SRB蛋白。

A mammalian SRB protein associated with an RNA polymerase II holoenzyme.

作者信息

Chao D M, Gadbois E L, Murray P J, Anderson S F, Sonu M S, Parvin J D, Young R A

机构信息

Whitehead institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

出版信息

Nature. 1996 Mar 7;380(6569):82-5. doi: 10.1038/380082a0.

DOI:10.1038/380082a0

PMID:8598913

Abstract

A large multisubunit complex containing RNA polymerase II, general transcription factors and SRB regulatory proteins initiates transcription of class II genes in yeast cells. The SRB proteins are a hallmark of this RNA polymerase II holoenzyme as they are found only in this complex, where they contribute to the response to regulators. We have now isolated a human homologue of the yeast SRB7 gene and used antibodies against human SRB7 protein to purify and characterize a mammalian RNA polymerase II holoenzyme containing the general transcription factors TFIIE and TFIIH. This holoenzyme is more responsive to transcriptional activators than core RNA polymerase II when assayed in the presence of coactivators.

摘要

一个包含RNA聚合酶II、通用转录因子和SRB调节蛋白的大型多亚基复合物启动酵母细胞中II类基因的转录。SRB蛋白是这种RNA聚合酶II全酶的一个标志，因为它们仅存在于该复合物中，在其中它们有助于对调节因子作出反应。我们现已分离出酵母SRB7基因的一个人类同源物，并使用针对人类SRB7蛋白的抗体来纯化和鉴定一种包含通用转录因子TFIIE和TFIIH的哺乳动物RNA聚合酶II全酶。在共激活因子存在的情况下进行测定时，这种全酶比核心RNA聚合酶II对转录激活因子的反应更灵敏。