Myers L C, Leuther K, Bushnell D A, Gustafsson C M, Kornberg R D
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Methods. 1997 Jul;12(3):212-6. doi: 10.1006/meth.1997.0473.
Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.
本文介绍了用于制备完全确定的酵母RNA聚合酶II转录系统的实验方案，该系统主要由纯化的TFIIB、-E、-F和-H、TATA结合蛋白、RNA聚合酶II以及转录调节介质组成。这个由44种多肽组成的系统能够在迄今为止测试的十几种酵母和哺乳动物启动子中的任何一个上起始转录，并对多种转录激活蛋白产生反应。
