V(D)J 重组起始与逆转录病毒整合之间的相似性。

Similarities between initiation of V(D)J recombination and retroviral integration.

作者信息

van Gent D C, Mizuuchi K, Gellert M

机构信息

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.

出版信息

Science. 1996 Mar 15;271(5255):1592-4. doi: 10.1126/science.271.5255.1592.

DOI:10.1126/science.271.5255.1592

PMID:8599117

Abstract

In the first step of V(D)J recombination, the RAG1 and RAG2 proteins cleave DNA between a signal sequence and the adjacent coding sequence, generating a blunt signal end and a coding end with a closed hairpin structure. These hairpins are intermediates leading to the formation of assembled antigen receptor genes. It is shown here that the hairpins are formed by a chemical mechanism of direct trans-esterification, very similar to the early steps of transpositional recombination and retroviral integration. A minor variation in the reaction is sufficient to divert the process from transposition to hairpin formation.

摘要

在V(D)J重排的第一步中，RAG1和RAG2蛋白在信号序列和相邻编码序列之间切割DNA，产生一个平端信号末端和一个具有封闭发夹结构的编码末端。这些发夹是导致组装抗原受体基因形成的中间体。本文表明，发夹是通过直接转酯的化学机制形成的，这与转座重排和逆转录病毒整合的早期步骤非常相似。反应中的一个微小变化就足以使该过程从转座转向发夹形成。