Melek M, Gellert M, van Gent D C
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.
Science. 1998 Apr 10;280(5361):301-3. doi: 10.1126/science.280.5361.301.
Assembly of immunoglobulin and T cell receptor genes from separate gene segments [V(D)J recombination] begins with DNA double-strand breakage by the RAG1 and RAG2 proteins, acting at a pair of recombination signal sequences (RSSs). Here, the RAG proteins are shown to reverse the cleavage reaction by joining an RSS to a broken coding sequence end. These "hybrid joints" have also been found in lymphoid cells, even when the normal pathway of DNA double-strand break repair is inactive, and can now be explained by this activity of the RAG proteins.
免疫球蛋白和T细胞受体基因由各自的基因片段组装而成（V(D)J重排），起始于RAG1和RAG2蛋白导致的DNA双链断裂，作用于一对重组信号序列（RSSs）。在此，RAG蛋白通过将一个RSS连接到断裂的编码序列末端，显示出逆转切割反应的能力。这些“杂交接头”在淋巴细胞中也已被发现，即使DNA双链断裂修复的正常途径不活跃，现在可以用RAG蛋白的这种活性来解释。
