Hoorfar J, Wedderkopp A
Danish Veterinary Laboratory, Copenhagen, Denmark.
Am J Vet Res. 1995 Dec;56(12):1549-54.
We investigated the ability of an antibody-specific, O antigen-based ELISA to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S typhimurium episodes within the previous 7 months, and 220 herds of unknown disease status, were tested. A herd was considered ELISA positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were ELISA positive, whereas all 8 herds with a known history of salmonellosis were ELISA positive herd specificity, 0.9 and herd sensitivity, 1.0). A significant correlation (P < 0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that ELISA testing of individual milk samples can be used for surveillance of herds for S typhimurium infections, but further modifications are needed to test bulk tank milk samples.
我们通过筛查牛奶样本，研究了一种基于抗体特异性、O抗原的酶联免疫吸附测定（ELISA）记录鼠伤寒沙门氏菌群体感染情况的能力。对三个牛群进行了检测，分别是20个无沙门氏菌病病史的牛群、8个在过去7个月内有鼠伤寒沙门氏菌发病史的牛群以及220个疾病状况不明的牛群。如果至少5%的奶牛OD值>0.3，则该牛群被视为ELISA阳性。在20个无沙门氏菌病病史的牛群中，只有2个牛群ELISA呈阳性，而所有8个有已知沙门氏菌病病史的牛群ELISA均呈阳性（群体特异性为0.9，群体敏感性为1.0）。在有沙门氏菌病病史的牛群中，奶牛血清和牛奶样本的OD值之间存在显著相关性（P<0.001）。得出的结论是，对单个牛奶样本进行ELISA检测可用于监测牛群的鼠伤寒沙门氏菌感染情况，但需要进一步改进以检测奶罐中的混合牛奶样本。
