Muro Y, Iwai T, Ohashi M
Department of Dermatology, Nagoya University School of Medicine, Japan.
Clin Immunol Immunopathol. 1996 Jan;78(1):86-9. doi: 10.1006/clin.1996.0013.
Autoantibodies against centromere proteins are commonly found in the serum of patients with scleroderma and other systemic autoimmune diseases. The reactivity of anticentromere autoantibodies (ACA) from 78 patients was investigated by ELISA using two kinds of a 15-amino-acid peptide corresponding to the N- and C-termini of CENP-A, one of the target molecules of ACA. The N-terminal peptide (residues 3-17) was recognized by 85% of ACA, while the C- terminal peptide (residues 126-140) was not. The ELISA result for the N-terminal peptide correlated with the immunoreactivity of CENP-A observed in immunoblotting. Moreover, the binding between autoantibodies and CENP-A was inhibited by the N-terminal peptide in 98.5% of anti-CENP-A- positive sera in immunoblotting. The sequence of peptide, PRRRSRKPEAPRRRS, is highly charged and has two repeats of PRRRS. These results indicate that the N-terminal-charged region forms a major epitope of CENP-A. This area may be involved in the induction of specific autoantibodies against centromere in autoimmune patients.
抗着丝粒蛋白自身抗体常见于硬皮病和其他系统性自身免疫性疾病患者的血清中。采用酶联免疫吸附测定（ELISA）法，使用两种分别对应于抗着丝粒自身抗体（ACA）的一种靶分子CENP - A的N端和C端的15个氨基酸的肽段，对78例患者的抗着丝粒自身抗体（ACA）的反应性进行了研究。85%的ACA能识别N端肽段（第3 - 17位氨基酸残基），而C端肽段（第126 - 140位氨基酸残基）则不能被识别。N端肽段的ELISA结果与免疫印迹中观察到的CENP - A的免疫反应性相关。此外，在免疫印迹中，98.5%的抗CENP - A阳性血清中的自身抗体与CENP - A之间的结合被N端肽段所抑制。肽段序列PRRRSRKPEAPRRRS带电量高，且有两个PRRRS重复序列。这些结果表明，N端带电区域构成了CENP - A的主要表位。该区域可能参与了自身免疫患者中抗着丝粒特异性自身抗体的诱导过程。
