Tenório F, Giraldi-Guimarães A, Mendez-Otero R
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
J Neurosci Res. 1995 Dec;42(5):633-7. doi: 10.1002/jnr.490420505.
We used the activity of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) to detect the presence of nitric oxide synthase (NOS) in the developing rat superior colliculus. Our results showed that NOS is present in cells and neuropil in the developing and adult rat superior colliculus. The first NOS-positive cells were detected at postnatal day 7 and were weakly stained. During the following days the number of stained cells increased markedly, reaching a peak by postnatal day 15, coinciding with the time of eye opening in the rat. By the end of the third postnatal week, both the number and intensity of stained cells showed an adult-like pattern. We conclude that NOS expression lags behind the initial period of reorganization of the retinotectal projection. However, NOS activity could be involved in the subsequent synaptic remodeling and plasticity of the retinocollicular projection.
我们利用烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）的活性来检测发育中的大鼠上丘中一氧化氮合酶（NOS）的存在。我们的结果表明，NOS存在于发育中和成年大鼠上丘的细胞和神经毡中。最早在出生后第7天检测到NOS阳性细胞，且染色较弱。在接下来的几天里，染色细胞的数量显著增加，在出生后第15天达到峰值，这与大鼠睁眼的时间一致。到出生后第三周结束时，染色细胞的数量和强度均呈现出类似成年动物的模式。我们得出结论，NOS的表达滞后于视网膜顶盖投射最初的重组期。然而，NOS活性可能参与了随后视网膜-上丘投射的突触重塑和可塑性。
