Radack K, Martin V, Wones R, Buncher R, Pinney S, Mandell K
Department of Medicine, University of Cincinnati School of Medicine, OH 45267-0535, USA.
Mutat Res. 1996 Mar 9;350(2):295-306. doi: 10.1016/0027-5107(95)00162-x.
The purpose of this study was to evaluate the intercorrelation between three genetic assays in 112 subjects. The group was pooled from two originally separate but homogeneous subgroups of 56 persons each. Procedures included assays for hprt mutant frequencies, micronuclei in human lymphocytes, and mutations at the glycophorin A (gpa) loci. We found no statistically significant or biologically important intercorrelations among the three biomarkers. We did, however, observe significant correlations between log(e) hprt mutant frequency and cloning efficiency (inverse correlation for these 2 variables), age and log(e) hprt mutant frequency, an inverse relationship between cloning efficiency and age, and an important differential sex effect favoring a greater micronuclei frequency in females than males. No significant correlations between the covariates of interest and glycophorin A variant frequencies NN or NO were observed. Using multivariable linear regression, age was found to account for the majority of the variability in hprt mutant frequency (greater than sex and/or smoking); for micronuclei data, only sex contributed a statistically significant and biologically important proportion to the total variation. We conclude that despite observing no significant intercorrelations between the three assays performed simultaneously from the same individuals in a large population database, a significant correlation between age and hprt mutant frequency and an inverse association between cloning efficiency and hprt do exist; furthermore, we verified the strong differential sex-specific effect on micronucleus frequencies.
本研究的目的是评估112名受试者中三种基因检测方法之间的相互关系。该组由两个最初分开但同质的亚组合并而成，每个亚组有56人。检测程序包括次黄嘌呤磷酸核糖转移酶（hprt）突变频率检测、人淋巴细胞微核检测以及血型糖蛋白A（gpa）位点突变检测。我们发现这三种生物标志物之间不存在统计学上显著或生物学上重要的相互关系。然而，我们确实观察到log(e) hprt突变频率与克隆效率之间存在显著相关性（这两个变量呈负相关）、年龄与log(e) hprt突变频率之间存在相关性、克隆效率与年龄之间呈负相关，以及存在重要的性别差异效应，即女性的微核频率高于男性。未观察到感兴趣的协变量与血型糖蛋白A变异频率NN或NO之间存在显著相关性。使用多变量线性回归发现，年龄占hprt突变频率变异性的大部分（大于性别和/或吸烟）；对于微核数据，只有性别对总变异有统计学上显著且生物学上重要的贡献。我们得出结论，尽管在一个大型人群数据库中，对同一批个体同时进行的三种检测之间未观察到显著的相互关系，但年龄与hprt突变频率之间确实存在显著相关性，克隆效率与hprt之间存在负相关；此外，我们证实了性别对微核频率有强烈的特异性差异效应。
