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食品诱变剂2-氨基-1-甲基-6-苯基咪唑并[4,5-β]吡啶（PhIP）及其代谢产物2-羟基氨基-1-甲基-6-苯基咪唑并[4,5-β]吡啶（N-OH-PhIP）对人及中国仓鼠细胞的体外细胞遗传学效应。

Cytogenetic effects of a food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (PhIP), and its metabolite, 2-hydroxyamino-1-methy-6-phenylimidazo[4,5-beta]pyridine (N-OH-PhIP), on human and Chinese hamster cells in vitro.

作者信息

Otsuka C, Miura K F, Satoh T, Hatanaka M, Wakabayashi K, Ishidate M

机构信息

Chromosome Research Center (CRC), Olympus Optical Co., Ltd., Tokyo, Japan.

出版信息

Mutat Res. 1996 Mar 1;367(3):115-21. doi: 10.1016/0165-1218(95)00082-8.

DOI:10.1016/0165-1218(95)00082-8
PMID:8600367
Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (PhIP) induced structural chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) in human lymphocytes and human diploid fibroblasts (TIG-7) at concentrations above 12.5 microgram /ml in the presence of rat S9 mix. PhIP also elevated the frequencies of SCEs in human lymphocytes in the presence of rat S9 at concentrations above 2.0 microgram/ml with dose-dependency. A proximate form of metabolites of PhIP, 2-hydroxy-amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (N-OH-PhIP), caused CAs in human and Chinese hamster fibroblast cells in the absence of S9 mix at concentrations above 0.75 microgram/ml and 1.25 microgram/ml, respectively, which were 10 times lower than the effective concentration of PhIP. No marked differences were observed in the cytogenetic sensitivity to N-OH-PhIP between human and Chinese hamster cells, except between lymphocytes obtained from different donors.

摘要

在大鼠S9混合物存在的情况下，2-氨基-1-甲基-6-苯基咪唑并[4,5-β]吡啶（PhIP）在浓度高于12.5微克/毫升时，可诱导人淋巴细胞和人二倍体成纤维细胞（TIG-7）出现结构染色体畸变（CAs）和姐妹染色单体交换（SCEs）。在大鼠S9存在的情况下，PhIP在浓度高于2.0微克/毫升时，也会使人类淋巴细胞中的SCEs频率升高，且具有剂量依赖性。PhIP的一种近似代谢产物，2-羟基氨基-1-甲基-6-苯基咪唑并[4,5-β]吡啶（N-OH-PhIP），在无S9混合物时，分别在浓度高于0.75微克/毫升和1.25微克/毫升时，可导致人和中国仓鼠成纤维细胞出现CAs，这一浓度比PhIP的有效浓度低10倍。除了来自不同供体的淋巴细胞外，人和中国仓鼠细胞对N-OH-PhIP的细胞遗传学敏感性未观察到明显差异。

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