Burns J W, Siadat-Pajouh M, Krishnaney A A, Greenberg H B
Department of Medicine, Stanford University School of Medicine, CA 94305, USA.
Science. 1996 Apr 5;272(5258):104-7. doi: 10.1126/science.272.5258.104.
Rotaviruses are the leading cause of severe gastroenteritis and dehydrating diarrhea in young children and animals worldwide. A murine model and "backpack tumor" transplantation were used to determine the protective effect of antibodies against VP4(an outer capsid viral protein) and VP6(a major inner capsid viral protein). Only two non-neutralizing immunoglobulin A (IgA) antibodies to VP6 were capable of preventing primary and resolving chronic murine rotavirus infections. These antibodies were not active, however, when presented directly to the luminal side of the intestinal tract. These findings support the hypothesis that in vivo intracellular viral inactivation by secretory IgA during transcytosis is a mechanism of host defense against rotavirus infection.
轮状病毒是全球范围内导致幼儿和动物严重肠胃炎及脱水腹泻的主要原因。利用小鼠模型和“背包肿瘤”移植来确定针对VP4（一种病毒外衣壳蛋白）和VP6（一种主要的病毒内衣壳蛋白）的抗体的保护作用。只有两种针对VP6的非中和性免疫球蛋白A（IgA）抗体能够预防原发性小鼠轮状病毒感染并解决慢性感染。然而，当这些抗体直接作用于肠道管腔侧时并无活性。这些发现支持了以下假说：在转胞吞过程中，分泌型IgA在体内对病毒进行细胞内灭活是宿主抵御轮状病毒感染的一种机制。
