一个高度甲基化的DNA岛与人类核糖体蛋白S14（RPS14）基因座中的一个染色体复制起点相关。

A densely methylated DNA island is associated with a chromosomal replication origin in the human RPS14 locus.

作者信息

Tasheva E S, Roufa D J

机构信息

Division of Biology, Kansas State University, Manhattan, Kansas, 66506, USA.

出版信息

Somat Cell Mol Genet. 1995 Nov;21(6):369-83. doi: 10.1007/BF02310205.

DOI:10.1007/BF02310205

PMID:8600566

Abstract

We describe a 258-bp densely methylated DNA island (DMI) and chromosomal origin of bidirectional DNA replication within the transcribed portion of the human RPS14 intron 1. Together with the DMIs previously detected in two functional Chinese hamster replication origins [see Ref. 1, pp. 5636-5644], observations described in this report strengthen the correlation between densely methylated DNA islands and active mammalian chromosomal replication origins. Accordingly, DMIs may prove to be reliable physical markers for origins of bidirectional DNA replication in complex genomic DNAs of higher animals.

摘要

我们描述了一个位于人类核糖体蛋白S14（RPS14）基因内含子1转录区域内的258碱基对的高度甲基化DNA岛（DMI）以及双向DNA复制的染色体起源。与之前在两个功能性中国仓鼠复制起点中检测到的DMI[见参考文献1，第5636 - 5644页]一起，本报告中描述的观察结果强化了高度甲基化DNA岛与活跃的哺乳动物染色体复制起点之间的相关性。因此，DMI可能被证明是高等动物复杂基因组DNA中双向DNA复制起点的可靠物理标记。