重症肌无力中抗乙酰胆碱受体自身抗体的检测
Measurement of anti-acetylcholine receptor auto-antibodies in myasthenia gravis.
作者信息
Steenkamp K J, Duim W, Myer M S, Malfeld S C, Anderson R
机构信息
Department of Immunology, University of Pretoria.
出版信息
S Afr Med J. 1995 Dec;85(12):1293-4.
Two different acetylcholine receptor (AChR) preparations derived from amputated human muscle (AChRAMP) and from the human rhabdomyosarcoma cell line TE671 (AChRTE671) were compared in radio-immunoprecipitation assays for the detection of AChR auto-antibodies in serum specimens from 20 patients with proven myasthenia gravis. Tests performed with the AChRTE671 and AChRAMP antigen preparations were positive in all the patients and in 19/20 respectively. A high degree of correlation (r = 0.94) was evident between the two auto-antigen preparations. Assays based on the use of TE671-derived antigen represent a useful alternative to the conventional assay using AChRAMP for the detection of AChR auto-antibodies.
在放射免疫沉淀试验中，对源自截肢人体肌肉的两种不同乙酰胆碱受体（AChR）制剂（AChRAMP）和源自人横纹肌肉瘤细胞系TE671的制剂（AChRTE671）进行了比较，以检测20例确诊为重症肌无力患者血清标本中的AChR自身抗体。使用AChRTE671和AChRAMP抗原制剂进行的检测在所有患者中均呈阳性，分别为19/20例。两种自身抗原制剂之间存在高度相关性（r = 0.94）。基于使用TE671衍生抗原的检测方法是使用AChRAMP进行传统检测以检测AChR自身抗体的一种有用替代方法。
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