Branca M, Culeddu N, Fruianu M, Serra M V
Dipartimento di Chimica, Università di Sassari, Sassari, Italy.
Anal Biochem. 1995 Nov 20;232(1):1-6. doi: 10.1006/abio.1995.9968.
We analyzed the changes in the shape and resonance positions of the 31P NMR signals of phospholipid mixtures in a single-phase solvent containing different quantities of chloroform, methanol, and water. We obtained extremely reproducible NMR signals when sufficient care was taken to control the desired solvent composition. The solvent composition can be predicted using a "tie line" in a ternary diagram of chloroform, methanol, and water or by mixing the three components at the desired ratio. Avoiding the presence of a biphasic system allows easy solvent preparation and NMR spectral reproducibility, with the possibility of complete recovery of the sample and absence of loses due to different repartition coefficients.
我们分析了磷脂混合物在含有不同量氯仿、甲醇和水的单相溶剂中的31P NMR信号的形状和共振位置变化。当采取足够的小心措施来控制所需的溶剂组成时，我们获得了极其可重复的NMR信号。溶剂组成可以使用氯仿、甲醇和水的三元相图中的“系线”来预测，或者通过按所需比例混合这三种组分来预测。避免双相系统的存在可使溶剂制备容易且NMR光谱具有可重复性，有可能完全回收样品且不存在因不同分配系数导致的损失。
