Kern T S, Engerman R L
Department of Ophthalmology and Visual Science, University of Wisconsin, Madison, 53706-1532, USA.
Arch Ophthalmol. 1996 Mar;114(3):306-10. doi: 10.1001/archopht.1996.01100130302013.
To isolate microvessels from cerebral cortex of dogs with alloxan-induced diabetes and dogs with experimental galactosemia to compare the prevalence of microvascular lesions in cerebral cortex with that in retina.
Microvessels were isolated from cerebral cortex of experimental animals using a sieving method, and compared with the retinal vasculature isolated from the same animals using the trypsin digestion method.
Dogs with diabetes or experimental galactosemia of 5 years' duration had retinopathy that was morphologically indistinguishable from that of humans with diabetes, including microaneurysms, acellular capillaries, and pericyte ghosts. These lesions never were seen in cerebral cortical vessels of the same animals. The only morphologic abnormality observed in cerebral capillaries of dogs with diabetes and dogs fed galactose was thickening of basement membrane.
Local influences in the eye apparently play an important role in the development of diabetic retinopathy. Current hypotheses about the pathogenesis of the retinopathy do not account adequately for such differences in the tissue distribution of vascular lesions.
从用四氧嘧啶诱导糖尿病的犬以及实验性半乳糖血症的犬的大脑皮层中分离微血管，以比较大脑皮层与视网膜中微血管病变的发生率。
采用筛分法从实验动物的大脑皮层中分离微血管，并与用胰蛋白酶消化法从同一动物分离的视网膜血管系统进行比较。
病程达5年的糖尿病犬或实验性半乳糖血症犬患有视网膜病变，其形态与糖尿病患者的视网膜病变无法区分，包括微动脉瘤、无细胞毛细血管和周细胞残影。在同一动物的大脑皮层血管中从未见过这些病变。在糖尿病犬和喂食半乳糖的犬的脑毛细血管中观察到的唯一形态学异常是基底膜增厚。
眼部的局部影响显然在糖尿病视网膜病变的发生中起重要作用。目前关于视网膜病变发病机制的假说不能充分解释血管病变在组织分布上的这种差异。
