A simple solid-state NMR method for determining peptidoglycan crosslinking in Bacillus subtilis.
作者信息
Shenouda N S, Pan Y, Schaefer J, Wilson G E
机构信息
Department of Chemistry, the University of Akron, OH, 44325, USA.
出版信息
Biochim Biophys Acta. 1996 Mar 15;1289(2):217-20. doi: 10.1016/0304-4165(95)00177-8.
PMID:8600976
Abstract
The crosslink index of the peptidoglycan of intact cell walls of Bacillus subtilis grown in media containing L-[15N]aspartic acid has been determined by a combination of cross-polarization magic-angle spinning 15N NMR and gas chromatography/mass spectrometry. The crosslink index of 62% decreased to 52% when the bacteria were exposed to the antibiotic cephalothin.
摘要
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