植物和人类中性粒细胞氧化爆发复合体含有免疫相关蛋白。

Plant and human neutrophil oxidative burst complexes contain immunologically related proteins.

作者信息

Dwyer S C, Legendre L, Low P S, Leto T L

机构信息

Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.

出版信息

Biochim Biophys Acta. 1996 Mar 15;1289(2):231-7. doi: 10.1016/0304-4165(95)00156-5.

DOI:10.1016/0304-4165(95)00156-5

PMID:8600979

Abstract

Generation of the microbicidal oxidative burst in human neutrophils requires participation of four proteins, a membrane bound flavocytochrome beta-558, two soluble proteins termed p47-phox and p67-phox, and the Ras-related GTPase Rac. Because plant cells exposed to pathogens produce a similar oxidative burst, we have looked for similarities between the oxidase complexes of the two systems. Antibodies against human neutrophil p47-phox and p67-phox were used to immunoblot cell extracts from several plant cell lines and were found to cross-react with proteins of the same molecular weight. Furthermore, plant cell lines not previously shown to produce an oxidative burst, yet found to express these immunoreactive proteins, rapidly generated hydrogen peroxide in response to elicitation. Finally, diphenylene iodonium (DPI) and alpha-naphthol, known specific inhibitors of the NADPH oxidase in neutrophils, also inhibited the oxidative burst in soybean cell suspensions with similar Ki values (about 15 microM and 30 microM respectively). These results provide evidence for involvement of proteins related to the neutrophil oxidase complex in the defense-related oxidative burst of plants.

摘要

人类中性粒细胞中杀菌性氧化爆发的产生需要四种蛋白质的参与，一种膜结合黄素细胞色素β-558、两种名为p47-吞噬氧化蛋白和p67-吞噬氧化蛋白的可溶性蛋白质，以及Ras相关GTP酶Rac。由于暴露于病原体的植物细胞会产生类似的氧化爆发，我们一直在寻找这两个系统的氧化酶复合物之间的相似性。针对人类中性粒细胞p47-吞噬氧化蛋白和p67-吞噬氧化蛋白的抗体被用于对几种植物细胞系的细胞提取物进行免疫印迹分析，结果发现它们与相同分子量的蛋白质发生交叉反应。此外，之前未显示会产生氧化爆发但发现表达这些免疫反应性蛋白质的植物细胞系，在受到诱导后会迅速产生过氧化氢。最后，二亚苯基碘鎓（DPI）和α-萘酚，已知是中性粒细胞中NADPH氧化酶的特异性抑制剂，也以相似的Ki值（分别约为15微摩尔和30微摩尔）抑制大豆细胞悬浮液中的氧化爆发。这些结果为与中性粒细胞氧化酶复合物相关的蛋白质参与植物防御相关的氧化爆发提供了证据。