使用固定化凝集素柱通过亲和色谱法对糖蛋白衍生的寡糖进行分级分离。

Fractionation of glycoprotein-derived oligosaccharides by affinity chromatography using immobilized lectin columns.

作者信息

Endo T

机构信息

Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, Japan.

出版信息

J Chromatogr A. 1996 Jan 12;720(1-2):251-61. doi: 10.1016/0021-9673(95)00220-0.

DOI:10.1016/0021-9673(95)00220-0

PMID:8601194

Abstract

Lectin affinity column chromatography is becoming a method of choice for the fractionation and purification of oligosaccharides, especially N-linked oligosaccharides. Using lectin affinity, it is easy to separate structural isomers and to isolate oligosaccharides based on specific features. Further, serial lectin column chromatography, when various lectin columns are used at the same time, can afford a very sensitive method for the fractionation and characterization of extremely small amounts of oligosaccharides. Thus, when used in conjunction with other separation techniques, lectin affinity chromatography can help to purify rapidly oligosaccharides and provide substantial information about their structural features.

摘要

凝集素亲和柱色谱法正成为寡糖尤其是 N - 连接寡糖分级分离和纯化的首选方法。利用凝集素亲和作用，很容易分离结构异构体并根据特定特征分离寡糖。此外，当同时使用各种凝集素柱时，串联凝集素柱色谱法可为极少量寡糖的分级分离和表征提供一种非常灵敏的方法。因此，当与其他分离技术结合使用时，凝集素亲和色谱法有助于快速纯化寡糖，并提供有关其结构特征的大量信息。