Linhardt R J, Pervin A
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City 52242-1112, USA.
J Chromatogr A. 1996 Jan 12;720(1-2):323-35. doi: 10.1016/0021-9673(95)00265-0.
Capillary electrophoresis (CE) has recently emerged as a highly promising technique consuming an extremely small amount of sample and capable of the rapid, high-resolution separation, characterization, and quantitation of analytes. CE has been used for the separation of biopolymers, including acidic carbohydrates. Since CE is basically an analytical method for ions, acidic carbohydrates that give anions in weakly acid, neutral, or alkaline media are often the direct objects of this method. The scope of this review is limited to the use of CE for the analysis of carbohydrates containing carboxylate, sulfate, and phosphate groups as well as neutral carbohydrates that have been derivatized to incorporate strongly acidic functionality, such as sulfonate groups.
毛细管电泳（CE）最近已成为一种极具前景的技术，它消耗的样品极少，能够对分析物进行快速、高分辨率的分离、表征和定量。CE已用于生物聚合物的分离，包括酸性碳水化合物。由于CE基本上是一种离子分析方法，在弱酸、中性或碱性介质中产生阴离子的酸性碳水化合物通常是该方法的直接分析对象。本综述的范围仅限于使用CE分析含有羧基、硫酸根和磷酸根的碳水化合物，以及已衍生化以引入强酸性官能团（如磺酸根）的中性碳水化合物。
