Storms S D, Anvekar V M, Adams L D, Murray B A
Department of Developmental and Cell Biology, University of California, Irvine 92717-2300, USA.
Exp Cell Res. 1996 Mar 15;223(2):385-94. doi: 10.1006/excr.1996.0093.
The vertebrate neural cell adhesion molecule NCAM mediates adhesion by both homophilic and heterophilic mechanisms, with heparan sulfate proteoglycans (HSPGs) being likely heterophilic ligands. In this study, transfected chicken NCAM polypeptides expressed on mouse L cells mediated the adhesion of these cells to several different heparan sulfate proteoglycans in nonionic detergent extracts of Embryonic Day 10 chicken brain membranes. In addition, adhesion inhibition experiments suggested a hitherto-undetected role for chondroitin sulfate proteoglycans in the stimulation of NCAM-mediated adhesion to some, but not all, of the HSPG ligands. Our experiments support the view that NCAM is a multivalent adhesive molecule whose function is affected by interactions with extracellular matrix and cell surface molecules.
脊椎动物神经细胞黏附分子NCAM通过同源和异源机制介导黏附，硫酸乙酰肝素蛋白聚糖（HSPG）可能是异源配体。在本研究中，在小鼠L细胞上表达的转染鸡NCAM多肽介导了这些细胞与胚胎第10天鸡脑膜非离子去污剂提取物中几种不同硫酸乙酰肝素蛋白聚糖的黏附。此外，黏附抑制实验表明，硫酸软骨素蛋白聚糖在刺激NCAM介导的对部分（而非全部）HSPG配体的黏附中具有迄今未被发现的作用。我们的实验支持这样一种观点，即NCAM是一种多价黏附分子，其功能受与细胞外基质和细胞表面分子相互作用的影响。
