Johnson S L, Weston J A
Institute of Neuroscience, University of Oregon, Eugene 97403, USA.
Genetics. 1995 Dec;141(4):1583-95. doi: 10.1093/genetics/141.4.1583.
When amputated, the fins of adult zebrafish rapidly regenerate the missing tissue. Fin regeneration proceeds through several stages, including wound healing, establishment of the wound epithelium, recruitment of the blastema from mesenchymal cells underlying the wound epithelium, and differentiation and outgrowth of the regenerate. We screened for temperature-sensitive mutations that affect the regeneration of the fin. Seven mutations were identified, including five that fail to regenerate their fins, one that causes slow growth during regeneration, and one that causes dysmorphic bumps or tumors to develop in the regenerating fin. reg5 mutants fail to regenerate their caudal fins, whereas reg6 mutants develop dysmorphic bumps in their regenerates at the restrictive temperature. Temperature-shift experiments indicate that reg5 and reg6 affect different stages of regeneration. The critical period for reg5 occurs during the early stages of regeneration before or during establishment of the blastema, resulting in defects in subsequent growth of the blastema and failure to differentiate bone-forming cells. The critical period for reg6 occurs after the onset of bone differentiation and during early stages of regenerative outgrowth. Both reg5 and reg6 also show temperature-sensitive defects in embryonic development or in ontogenetic outgrowth of the juvenile fin.
成年斑马鱼的鳍被截断后，缺失的组织能迅速再生。鳍的再生过程经历几个阶段，包括伤口愈合、伤口上皮的形成、从伤口上皮下方的间充质细胞募集再生芽基，以及再生组织的分化和生长。我们筛选了影响鳍再生的温度敏感突变。鉴定出七个突变，其中五个无法再生鳍，一个在再生过程中导致生长缓慢，还有一个在再生的鳍中导致发育异常的肿块或肿瘤形成。reg5突变体无法再生其尾鳍，而reg6突变体在限制温度下其再生组织中会出现发育异常的肿块。温度转换实验表明，reg5和reg6影响再生的不同阶段。reg5的关键时期发生在再生芽基形成之前或形成过程中的再生早期，导致再生芽基随后生长缺陷以及无法分化成骨细胞。reg6的关键时期发生在骨分化开始后以及再生生长的早期阶段。reg5和reg6在胚胎发育或幼鱼鳍的个体发育生长中也表现出温度敏感缺陷。
