Bode-Lesniewska B, Dours-Zimmermann M T, Odermatt B F, Briner J, Heitz P U, Zimmermann D R
Institute of Clinical Pathology, Department of Pathology, University of Zürich, Switzerland.
J Histochem Cytochem. 1996 Apr;44(4):303-12. doi: 10.1177/44.4.8601689.
We studied the distribution of the large hyaluronan-binding proteoglycan versican (also known as PG-M) in human adult tissues using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants VO and V1. Versican was present in the loose connective tissues of various organs and was often associated with the elastic fiber network. Furthermore, it was localized in most smooth muscle tissues and in fibrous and elastic cartilage. Versican staining was also noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. In blood vessels, versican was present in all three wall layers of veins and elastic arteries. In muscular arteries the immunoreactivity was normally restricted to the tunica adventitia. However, it appeared in the media and the split elastica interna of atherosclerotically transformed vessel walls. Our survey of the distribution of versican in normal human tissues now forms the basis for extended studies of potentially aberrant versican expression during pathogenic processes.
我们使用亲和纯化的多克隆抗体研究了大型透明质酸结合蛋白聚糖多功能蛋白聚糖（也称为PG-M）在成人组织中的分布，该抗体可识别多功能蛋白聚糖主要剪接变体VO和V1的核心蛋白。多功能蛋白聚糖存在于各种器官的疏松结缔组织中，并且经常与弹性纤维网络相关。此外，它定位于大多数平滑肌组织以及纤维软骨和弹性软骨中。在中枢和外周神经系统、表皮基底层以及一些腺上皮的腔表面也发现了多功能蛋白聚糖染色。在血管中，多功能蛋白聚糖存在于静脉和弹性动脉的所有三层壁中。在肌性动脉中，免疫反应性通常局限于外膜。然而，它出现在动脉粥样硬化转化血管壁的中膜和分裂的内弹性膜中。我们对正常人体组织中多功能蛋白聚糖分布的调查现在为进一步研究致病过程中可能异常的多功能蛋白聚糖表达奠定了基础。
