Dupuis F, Denizot Y, Fixe P, Dulery C, Praloran V
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Biochim Biophys Acta. 1996 Mar 27;1311(1):27-32. doi: 10.1016/0167-4889(95)00193-x.
Platelet-activating factor (PAF), a phospholipid autacoid with potent regulatory functions, is synthesized by stimulated monocytes. Macrophages are a source of the plasma acetylhydrolase activity (AHA) which regulates PAF concentrations. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) are involved in the differentiation and functions of cells from the monocytic/macrophagic lineage. This work reports that M-CSF and GM-CSF stimulated AHA production by human blood monocyte-derived macrophages in a time- and dose-dependent manner. After 7 days of culture without serum, a 6- and 4-fold increase was found in cells treated with M-CSF (1000 U/ml) and GM-CSF (50 ng/ml), respectively. M-CSF (up to 1000 U/ml) and GM-CSF (up to 10 ng/ml) did not induce PAF production by human blood monocytes. While GM-CSF (10 ng/ml) and interleukin-1 (10 U/ml) stimulated M-CSF production from monocyte-derived macrophages, PAF did not. These results indicate that M-CSF and GM-CSF enhance AHA production by human blood-derived macrophages cultured in low serum concentrations. Clearly the effects of growth factors on AHA production in vivo deserve to be assessed.
血小板活化因子（PAF）是一种具有强大调节功能的磷脂自分泌物质，由受刺激的单核细胞合成。巨噬细胞是调节PAF浓度的血浆乙酰水解酶活性（AHA）的来源。粒细胞-巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞集落刺激因子（M-CSF）参与单核细胞/巨噬细胞谱系细胞的分化和功能。这项研究报告称，M-CSF和GM-CSF以时间和剂量依赖的方式刺激人血单核细胞衍生巨噬细胞产生AHA。在无血清培养7天后，用M-CSF（1000 U/ml）和GM-CSF（50 ng/ml）处理的细胞分别增加了6倍和4倍。M-CSF（高达1000 U/ml）和GM-CSF（高达10 ng/ml）不会诱导人血单核细胞产生PAF。虽然GM-CSF（10 ng/ml）和白细胞介素-1（10 U/ml）刺激单核细胞衍生巨噬细胞产生M-CSF，但PAF不会。这些结果表明，M-CSF和GM-CSF可增强低血清浓度培养的人血源巨噬细胞的AHA产生。显然，生长因子对体内AHA产生的影响值得评估。
