Fujimoto M, Kanzaki H, Nakayama H, Higuchi T, Hatayama H, Iwai M, Kaneko Y, Mori T, Fujita J
Department of Gynecology and Obstetrics, Kyoto University, Kyoto, Japan.
Endocrinology. 1996 Mar;137(3):1096-101. doi: 10.1210/endo.137.3.8603579.
Differentiation of endometrial stromal cells (decidualization) is essential for embryo implantation and maintenance of pregnancy. By sequential complementary DNA subtractive hybridization, one of the messenger RNAs (mRNA) induced by progesterone in human endometrial stromal cells decidualized in vitro was identified as that of a tissue transglutaminase type II (TGase). TGase mRNA was induced within 6 h after the addition of progesterone to the culture, and the effect was dose dependent. Both the TGase inhibitor monodansylcadaverine and oligodeoxynucleotide complementary to the TGase mRNA inhibited the decidualization, as assessed by PRL production and morphological transformation. Expression of TGase mRNA in human decidua and endometria exposed to high levels of progesterone in vivo was demonstrated by Northern blotting and in situ hybridization. These data suggest that TGase is necessary for the decidualization of human endometrial stromal cells and that clarification of the mechanism of action of TGase will facilitate further insight into the diagnosis and treatment of infertility.
子宫内膜基质细胞的分化（蜕膜化）对于胚胎着床和维持妊娠至关重要。通过连续的互补DNA消减杂交，在体外蜕膜化的人子宫内膜基质细胞中，一种由孕酮诱导的信使核糖核酸（mRNA）被鉴定为组织转谷氨酰胺酶II型（TGase）的mRNA。在向培养物中添加孕酮后6小时内，TGase mRNA被诱导，且该效应呈剂量依赖性。通过催乳素分泌和形态转化评估，TGase抑制剂单丹磺酰尸胺和与TGase mRNA互补的寡脱氧核苷酸均抑制蜕膜化。通过Northern印迹法和原位杂交法证实了TGase mRNA在体内暴露于高水平孕酮的人蜕膜和子宫内膜中的表达。这些数据表明，TGase对于人子宫内膜基质细胞的蜕膜化是必需的，并且阐明TGase的作用机制将有助于进一步深入了解不孕症的诊断和治疗。
