Harp J A, Sacco R E
US Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010-0070, USA.
J Parasitol. 1996 Apr;82(2):245-9.
Lymphocyte phenotypes and cellular immune responses (blastogenesis and production of cytokines) to Cryptosporidium parvum were determined for spleen cells taken from BALB/c mice. These parameters were measured in mice at 1, 2, 3, and 4 wk of age, either exposed or not exposed to C. parvum in vivo. The percentage of T cells and the T-helper subset increased from weeks 2 to 4; B cells reached a peak percentage at 2 wk. Blastogenic responses were elevated at 1 wk and declined to a low level during weeks 2 to 4. Interferon-gamma production was maximal at 4 wk. No interleukin-5 production was seen. Data obtained were similar for cells from mice either exposed or not exposed to C. parvum in vivo. These data indicate that age-related changes, particularly the increased percentage of T cells and increased interferon-gamma production, are temporally related to the acquisition of resistance to colonization of mice with C. parvum. The data also indicate that these age-related changes occur in the absence of specific exposure to parasite antigens.
对取自BALB/c小鼠的脾细胞，测定了其淋巴细胞表型以及针对微小隐孢子虫的细胞免疫反应（增殖反应和细胞因子产生）。在1、2、3和4周龄的小鼠体内，无论是否暴露于微小隐孢子虫，均对这些参数进行了测量。T细胞和辅助性T细胞亚群的百分比从第2周增加至第4周；B细胞在第2周达到百分比峰值。增殖反应在第1周升高，在第2至4周降至低水平。γ干扰素的产生在第4周达到最大值。未观察到白细胞介素-5的产生。对于体内暴露或未暴露于微小隐孢子虫的小鼠的细胞，所获得的数据相似。这些数据表明，与年龄相关的变化，尤其是T细胞百分比的增加和γ干扰素产生的增加，在时间上与小鼠获得对微小隐孢子虫定植的抵抗力相关。数据还表明，这些与年龄相关的变化在没有特异性暴露于寄生虫抗原的情况下也会发生。
