Soulez M, Tuil D, Kahn A, Gilgenkrantz H
ICGM, U129 INSERM, Paris, France.
Biochem Biophys Res Commun. 1996 Feb 15;219(2):418-22. doi: 10.1006/bbrc.1996.0248.
CArG boxes, whose consensus sequence is CC(A/T)6GG, are involved in two very different types of transcriptional responses: response of immediate early genes to serum, mediated by so-called Serum Response Elements (SRE), and transcriptional activation of muscle-specific genes during muscle differentiation. Although previous studies have shown that the Serum Response Factor (SRF) binds to muscular CArG boxes, the role of such a binding in muscle-specific activation of CArG box-dependent genes was not directly demonstrated. Here, by transient co-transfection experiments, we demonstrate that intact SRF is required for muscle-specific transcriptional activation through CArG boxes.
CArG 框的共有序列为 CC(A/T)6GG，它参与两种截然不同的转录反应：即由所谓的血清反应元件（SRE）介导的早期即刻基因对血清的反应，以及肌肉分化过程中肌肉特异性基因的转录激活。尽管先前的研究表明血清反应因子（SRF）可与肌肉 CArG 框结合，但这种结合在 CArG 框依赖性基因的肌肉特异性激活中的作用尚未得到直接证实。在此，通过瞬时共转染实验，我们证明完整的 SRF 是通过 CArG 框进行肌肉特异性转录激活所必需的。
