人双侧灌注胎盘小叶中α-氨基异丁酸转运的钙依赖性

Calcium dependence of alpha-aminoisobutyric acid transfer in human bilaterally perfused placental lobules.

作者信息

Krishna B R, Gude N M, King R G, Brennecke S P

机构信息

Monash Perinatal Unit, Monash Medical Center, Clayton, Australia.

出版信息

J Perinat Med. 1995;23(5):343-51. doi: 10.1515/jpme.1995.23.5.343.

DOI:10.1515/jpme.1995.23.5.343

PMID:8606339

Abstract

Human isolated bilaterally perfused placental lobules were employed to study the Ca++ dependence of materno-fetal transfer of the non-metabolisable amino acid, alpha-aminoisobutyric acid (AIB). Bilateral perfusion with Ca(++)-free Krebs' solution containing 2 mmol/l EDTA for 60 min resulted in a significant reduction in materno-fetal AIB transfer compared to controls perfused with normal Krebs' solution. However, bilateral perfusion with Krebs' solution containing: 2.5 mmol/l Ca++ and 2 mmol/l EDTA; 3.8 mmol/l Ca++ with no EDTA; or Ca(++)-free Krebs' solution alone did not produce significantly different materno-fetal AIB transfer compared to placentae perfused with normal Krebs' solution. The reduction in AIB transfer during perfusion with Ca(++)-free Krebs' solution with EDTA was partially restored with subsequent reperfusion with normal Krebs' solution for 90 min. These results suggest that AIB transfer in human perfused placental lobules is at least partially Ca(++)-dependent.

摘要

使用人离体双侧灌注的胎盘小叶来研究不可代谢氨基酸α-氨基异丁酸（AIB）母胎转运对钙离子的依赖性。与用正常 Krebs 溶液灌注的对照组相比，用含 2 mmol/L EDTA 的无钙 Krebs 溶液双侧灌注 60 分钟导致母胎 AIB 转运显著降低。然而，与用正常 Krebs 溶液灌注的胎盘相比，用含 2.5 mmol/L 钙离子和 2 mmol/L EDTA 的 Krebs 溶液、不含 EDTA 的 3.8 mmol/L 钙离子的 Krebs 溶液或单独的无钙 Krebs 溶液双侧灌注并未产生显著不同的母胎 AIB 转运。在用含 EDTA 的无钙 Krebs 溶液灌注期间 AIB 转运的降低在随后用正常 Krebs 溶液再灌注 90 分钟后部分恢复。这些结果表明，人灌注胎盘小叶中的 AIB 转运至少部分依赖于钙离子。